What Is an In Vitro Skin Corrosion Test?

The In Vitro Skin Corrosion Test is one of the advanced cell culture-based safety tests conducted to evaluate the potential of a substance or product to cause irreversible tissue damage on the skin. In these tests, the corrosive effects of products are evaluated in a laboratory setting using Reconstructed Human Epidermis (RhE) models that mimic the biological structure of human skin.

Widely used in safety assessments of chemical substances, biocidal products, cosmetic raw materials, medical devices, and industrial products, this method is a key component of modern testing strategies developed as alternatives to animal testing.

At NanoTox, we offer reliable and scientifically validated In Vitro Skin Corrosion testing services within our In Vitro Cell Culture Unit, in accordance with international standards.

Purpose of the In Vitro Skin Corrosion Test

This test aims to determine whether a product or substance may cause irreversible damage to human skin.

Through these studies:

• The potential for corrosive effects is determined,
• Product safety is evaluated,
• Chemical classification processes are supported,
• Regulatory requirements are met,
• Scientific data is provided for product development efforts.

Reliable Assessment with the RhE Model

RhE (Reconstructed Human Epidermis) models used in In Vitro Skin Corrosion tests are three-dimensional cell culture systems that mimic the structural and functional characteristics of human epidermis.

Thanks to these models:

• Results close to those of human skin are obtained,
• Alternative methods to animal testing are applied,
• Reliable and reproducible data is generated,
• Internationally recognized test results are provided.

Studies Compliant with OECD Guidelines

In Vitro Skin Corrosion tests conducted at NanoTox are carried out in accordance with internationally recognized OECD guidelines.

Primary standards used:

• OECD 431 – In Vitro Skin Corrosion: Reconstructed Human Epidermis Test Method
• OECD 430 – Transcutaneous Electrical Resistance Test Method

are known as such.

These methods ensure the reliable determination of a product’s corrosive properties.

Test Principle

The test substance is applied to the RhE model at specified intervals. Following exposure, cell viability is assessed to determine whether the product causes a corrosive effect.

The following are frequently examined in studies:

• Cell viability analyses,
• MTT measurements,
• Tissue integrity assessments,
• Quality control parameters

are examined.

Based on the results obtained, the product can be classified as corrosive.

Reliable Cell Culture Studies with NanoTox

NanoTox conducts In Vitro Skin Corrosion tests in accordance with international standards using modern cell culture laboratories and a team of experts. We ensure the reliable acquisition of scientific data required for product safety and regulatory processes.

For detailed information about In Vitro Skin Corrosion tests and our other cell culture services, please contact NanoTox.