What Is the In Vitro Skin Irritation (RhE) Test?

The In Vitro Skin Irritation (RhE) test is one of the advanced cell culture-based testing methods used to assess the potential of a substance or product to cause skin irritation. These tests utilize Reconstructed Human Epidermis (RhE) models, which biologically mimic human skin.

Widely used in safety assessments of cosmetic products, chemicals, biocidal products, medical devices, and raw materials, this method is a key component of alternative testing strategies that reduce the use of animals.

At NanoTox, we offer reliable and scientifically validated In Vitro Skin Irritation (RhE) testing services within our In Vitro Cell Culture Unit, in accordance with international standards.

Purpose of the In Vitro Skin Irritation (RhE) Test

This test aims to evaluate, in a laboratory setting, whether a product or substance may cause irritation on human skin.

Through these studies:

• The potential for skin irritation is determined,
• Product safety is assessed,
• Regulatory requirements are met,
• Risk assessment processes are supported,
• Product development efforts are accelerated.

What Is the RhE Model?

RhE (Reconstructed Human Epidermis) are three-dimensional cell culture models that mimic the structure and biological properties of human epidermis.

Thanks to these models;

• Results close to those of human skin are obtained,
• More ethically sustainable testing processes are implemented,
• Reliable data serving as an alternative to animal testing is provided.

Today, RhE models are among the standard testing systems recognized by many international authorities.

Studies Compliant with OECD Guidelines

In Vitro Skin Irritation (RhE) tests conducted at NanoTox are performed in accordance with internationally recognized standards.

The primary guideline used is:

• OECD 439 – In Vitro Skin Irritation: Reconstructed Human Epidermis Test Method

as it is known.

This method ensures the reliable and reproducible assessment of a product’s skin irritation potential.

Test Principle

In the In Vitro Skin Irritation (RhE) test, the test substance is applied to a reconstructed human epidermis model at specific intervals. Following exposure, cell viability is analyzed to assess the product’s irritation potential.

The following are frequently examined in these studies:

• Cell viability measurements,
• MTT assays,
• Tissue integrity assessments,
• Quality control parameters

are examined.

Reliable Cell Culture Studies with NanoTox

NanoTox conducts In Vitro Skin Irritation (RhE) tests in accordance with international standards using modern cell culture laboratories and a team of experts. We ensure the reliable acquisition of scientific data required for product safety, biocompatibility, and regulatory processes.

For detailed information about In Vitro Skin Irritation (RhE) tests and our other cell culture services, please contact NanoTox.