What Is a Cytotoxicity Test?

A cytotoxicity test is one of the fundamental in vitro safety tests conducted to evaluate the toxic effects of a substance, product, or biomaterial on living cells. These cell culture-based studies are of critical importance in the biological safety assessments of medical devices, pharmaceuticals, biomaterials, cosmetic raw materials, biocidal products, and chemical substances.

At NanoTox, we offer cytotoxicity testing services in accordance with international standards, utilizing our modern cell culture laboratories and quality systems compliant with GLP (Good Laboratory Practice) principles.

The Purpose of Cytotoxicity Testing

Cytotoxicity tests are conducted to identify potential adverse effects of a substance on cell viability, cell growth, and cellular functions.

Through these studies,

• product safety assessments,
• biocompatibility studies,
• medical device development processes,
• chemical risk assessments,
• and regulatory and licensing applications

are supported by the necessary scientific data.

Why Are Cytotoxicity Tests Important?

Evaluating a product’s safety at the cellular level before it comes into contact with the human body is of great importance. Cytotoxicity tests enable the early detection of potential cell damage, thereby offering time and cost advantages in product development processes.

Additionally, these studies are recognized as one of the initial stages of biological safety assessments.

Studies Conducted in Compliance with ISO Standards

Cytotoxicity tests conducted at NanoTox are carried out in accordance with internationally recognized standards. The primary standards used include:

• ISO 10993-5 – Tests for In Vitro Cytotoxicity
• ISO 10993-12 – Sample Preparation and Reference Materials

are included.

Results obtained in accordance with these standards provide reliable data accepted in global regulatory processes.

Methods Used in Cytotoxicity Tests

Depending on the characteristics of the product and material, different cytotoxicity assessment methods can be applied. These include:

• MTT Test
• XTT Assay
• Neutral Red Uptake (NRU) Assay
• Cell Viability Analyses
• Direct Contact Method
• Extract Method

are included.

The selection of the appropriate method is determined based on the product type and relevant standard requirements.

Parameters Evaluated in Cytotoxicity Studies

Various cellular parameters are evaluated in detail throughout the test:

• Cell viability,
• Cell proliferation,
• Morphological changes,
• Inhibition of cell growth,
• Indicators of cellular damage,
• Dose-response relationships

Thanks to these comprehensive evaluations, the biological effects of the product on cells are analyzed in detail.

Reliable Cell Culture Studies with NanoTox

NanoTox prioritizes scientific accuracy, quality, and reliability in cytotoxicity tests conducted within the In Vitro Cell Culture Unit. With our experienced team of experts and modern laboratory infrastructure, we provide the reliable data you need for the biological safety assessments of your products.

For detailed information about cytotoxicity tests and our other cell culture services, please contact NanoTox.